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Available methods for deeper super-resolution imaging in plants require specialized hardware or fluorescent reagents. Here, the authors report a dynamic, deep-tissue single-molecule bioimaging technology and show its application in tracking two vernalization-specific proteins with which Arabidopsis forms memory of winter cold.

BioID detection of Ebola virus–host interactions when computationally integrated with existing host protein-protein networks, revealed functional cell protein complexes supporting infection, including the RNA decapping complex.

Betaglycan is a co-receptor for selective TGF-β family ligands. Here, the authors solve its structure in complex with TGF-β and the signaling receptors, which explains its ligand selectivity and reveals its mechanism in potentiating TGF-β signaling.

Using SCN2A haploinsufficiency as a proof-of-concept, upregulation of the existing functional gene copy through CRISPR activation was able to rescue neurological-associated phenotypes in Scn2a haploinsufficient mice and human neurons.

Here they generate endogenous optogenetic RhoGEFs and use them to quantitatively direct and study epithelial furrowing via cell shortening, uncovering design principles for morphogenetic furrowing including a role for tissue mechanics in furrow asymmetry.

Immune cells are believed not to generate large traction forces during migration. Now, measurements of natural killer cells in dense tissue reveal bursts of large traction forces as they move through narrow pores.

Here it is shown that the handedness of mirror-image flowers in Cyanella alba is not genetically determined but linked to the spiral arrangement of leaves and asymmetric auxin activity. This reveals a non-genetic mechanism for floral asymmetry.

The interconversion of spin and charge is fundamental to the operation of spintronic devices. Here the authors demonstrate spin-to-charge conversion in the correlated material vanadium dioxide, and show that the efficiency changes dramatically across the metal-insulator transition.

This study refines benthic foraminiferal Mg/Ca paleothermometry to reduce uncertainties to ±0.2-0.3 °C, which was then applied in Atlantic and Pacific sediment cores to reconstruct Mid-Pliocene M2 glaciation sea-level changes.

Vascularization remains a significant challenge in organoid technology. Here, the authors develop a microfluidic platform that enhances organoid growth, function and maturation, by establishing functional perfusable vascular networks.

In Arabidopsis thaliana, downregulation of the floral repressor FLC in response to cold occurs through a mechanism in which the FLC activator FRIGIDA is sequestered into biomolecular condensates away from the FLC promoter.

Though organic light-emitting diodes (OLEDs) with impressive flexibility have been reported, achieving stable operation in air and moist environments remains difficult. Here, the authors report ultrathin OLEDs with hybrid thin-film encapsulation barriers for stable operation in harsh environments.

Probing endogenous protein localization and function in vivo remains challenging due to laborious gene targeting and monofunctional alleles. Here, using a toolkit consisting of genetically-encoded epitope probes, their cognate tags, and an array of adapter proteins, the authors describe a methodology that enables visualization and manipulation of endogenous proteins in vertebrate systems.

A technique called LICONN (light-microscopy-based connectomics) allows mapping of brain tissue at synapse level and simultaneous measurement of molecular information, thus enabling quantification of cellular properties and multimodal analysis of brain tissue.

When injured, fish release an alarm substance produced by club cells in the skin that elicits fear in members of their shoal. Here, the authors show that mucus and bacteria are transported from the external surface into club cells, and bacterial components elicit alarm behaviour, acting in concert with a substance from fish.

Antisense transcription from genic regions is a common phenomenon. Here Rosa et al. use single molecule FISH to show that during vernalization in Arabidopsis, cold-induced antisense transcription of COOLAIR is mutually exclusive to sense-strand transcription of the flowering regulator FLCfrom the same loci.

Cagrilintide is a long-acting agonist of amylin and calcitonin receptors in late phase trials for obesity. Here, authors present structures of cagilintide with each target receptor, revealing the molecular basis for its non-selective action.

Jessop et. al. investigate the expression, activity, structure and supramolecular assembly of the arginine decarboxylase from Providencia stuartii, compare its polymers with those formed by the Escherichia coli lysine decarboxylase, and analyse the evolutionary conservation of the structural determinants of the polymerisation of these enzymes in enterobacteria.

The mechanisms of neurovascular adaptations underlying stress resilience remain unclear. Here the authors show that the astrocytic endocannabinoid system modulates the blood–brain barrier changes during stress in adult mice.

Cryopreservation is standard protocol prior to using NK cells in immunotherapy. Here the authors show that cryopreservation substantially reduces the clinical utility of these cells owing to a defect in their motility, an effect that might account for failure to treat some cancers with NK cell immunotherapy.

Deafness is the most common form of sensory impairment in humans and frequently caused by defects in hair cells of the inner ear. Here authors demonstrate that in a mouse model for recessive non-syndromic deafness (DFNB6), inactivation of Tmie in hair cells disrupts gene expression in the neurons that innervate them, suggesting treatment modalities for deafness may require restoration of the function of both hair cells and neurons.

Organic LEDs (OLEDs) have generally been considered to be slow devices. Through engineering the structure and materials of OLEDs, the authors achieve a breakthrough in the high-speed operation of OLEDs and demonstrate a 1 Gbps optical wireless link using the OLEDs.

The vascular, cellular and molecular changes underlying sex differences in mood disorders are unclear. Here, the authors show that blood-brain barrier dysfunction modulates anxiety- and depressive-like behaviors in female mice and endothelium-specific changes associated with maladaptive responses compared to resilience to stress.

This study describes ultrastructural changes and cell-specific calcium responses that accompany cytoplasmic bridge formation in legume root cells committed to symbiotic rhizobial infection, with fine-tuning by a calcium-binding annexin.

Differences in virulence across the Listeria monocytogenes species are determined by the fine-tuning of SigB pathway responsiveness and reflect host adaptation.

Dysregulated CD4 T cells have been implicated in autoimmune liver disease, but their phenotypes and origin are still unclear. Here the authors profile circulating, autoreactive CD4 T cells to find transcription signatures similar to tissue-activated, exhausted T cells, thereby hinting a tissue origin for these circulation CD4 T cells.

Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.

Nascent non-coding RNA can mediate chromatin silencing, however mechanistically this process is poorly understood. Here the authors show that resolution of an R-loop during 3'-end processing of a plant antisense transcript recruits chromatin modifiers to promote chromatin silencing.

Prediction of Unseen Proteins’ Subcellular localization (PUPS) combines a protein language model and an image inpainting model to utilize both protein sequence and cellular images for predicting protein localization on unseen proteins in a way that captures single-cell variability and cell-type specificity.

Morphological and electrophysiological understanding of cerebrospinal fluid-contacting neurons (CSF-cNs) is extended by analysis in the mouse, revealing that they express PKD2L1 and ASICs along with ligand- and voltage-gated channels modulated by metabotropic receptors.

Rzemieniewski et al. demonstrated that Arabidopsis thaliana CEPs regulate immunity via three CEP receptors with distinct expression patterns and ligand specificities. CEPs and their receptors coordinate nitrogen availability with immune responses.

A universal mechanical exfoliation method of creating freestanding membranes of complex-oxide materials with different crystal structures and orientations and stacking them to produce a range of artificial heterostructures with hybridized physical properties is described.

Determination of the high-resolution structure of yeast TORC1 allows characterization of the precise interfaces of interaction between inactive TORC1 and TORC1′ polymers and identification of the mode of binding of active EGOC on TORC1.

The study of spin orbit torques in insulating materials via conventional transport methods is restricted due to low electrical conductivity. Here, the authors use magneto-optical methods to measure spin orbit torques in ferromagnetic-insulator/heavy-metal heterostructures.

Natural Killer cells regulate foetal growth. Here the authors use a humanized transgenic mouse to demonstrate that specific HLA-C KIR2DL interactions promote changes in maternal and foetal cell transcriptomes, resulting in modifications to placental vasculature, intercellular communications and foetal growth restriction.

A dataset of 3D images from more than 200,000 human induced pluripotent stem cells is used to develop a framework to analyse cell shape and the location and organization of major intracellular structures.

Specific ssDNA binding proteins promote the annealing of complementary strands. Here the authors reveal an intermediate of annealing in which a duplex DNA is bound in an unusual conformation that is highly extended and unwound for the ssDNA binding protein Redβ from bacteriophage λ.

Whipworms are large parasites causing chronic disease in humans and other mammals. Here, the authors show how larvae create tunnels inside the gut lining and reveal the early host response to infection via Isg15 in mice and murine caecaloids.

Our understanding on the humoral immunity induced by SARS-CoV-2 is still lacking. Here the authors analyze B cell responses at the single cell level to find that, in severe COVID-19 patients, plasmablasts shift from IFN to TGFβ instruction to produce IgA antibodies that are not specific to dominant SARS-CoV-2 antigens.

A γδ T cell–IL-3 signalling axis is defined that controls the allergen responsiveness of cutaneous sensory neurons, leading to evidence for an immune rheostat that governs sensory neuronal responses to allergens on first exposure.

Gouwens et al. established a morpho-electrical taxonomy of cell types for the mouse visual cortex via unsupervised clustering analysis of multiple quantitative features from 1,938 neurons available online at the Allen Cell Types Database.

Using a range of laboratory and synchrotron techniques, Hickman-Lewis et al. show that some of Earth’s earliest siliciclastic microbial mat fossils (2.9 Ga) unexpectedly preserve biogeochemical signatures in nanoscale domains of organic material.

Large language models can be manipulated to generate misinformation by poisoning of a very small percentage of the data on which they are trained, but a harm mitigation strategy using biomedical knowledge graphs can offer a method for addressing this vulnerability.

Endosomal biogenesis is vital for eukaryotic cell physiology and relies on membrane fusion promoted by the CORVET tethering complex. Here, the authors solved the structure of CORVET by cryo-EM and reveal its minimal requirements for membrane tethering.

The typical electronic modulation of terahertz quantum cascade lasers is fundamentally limited at fast timescales by device properties. Here the authors propose and study an alternative, acoustic mechanism for modulating such THz QCLs at high speed.

The developmental mechanisms responsible for the structural defects observed in hypoplastic left heart syndrome remain controversial. Using rbfox-deficient zebrafish, the authors implicate impaired cardiac function as a primary driver of disease.

Acetylcholine signaling by the parasympathetic nervous system is crucial for proper insulin release. Alejandro Caicedo and his colleagues now show that such cholinergic signaling in human pancreatic islets is instead locally derived by pancreatic alpha cells—a finding that may have an impact on future drug development to treat diabetes.

IgG glycosylation is an important factor in immune function, yet the molecular details of protein glycosylation remain poorly understood. The data-driven approach presented here uses large-scale plasma IgG mass spectrometry measurements to infer new biochemical reactions in the glycosylation pathway.

Towards the end of their lives, stars like the Sun expand greatly to become red giant stars that oscillate. Such evolved stars could provide stringent tests of stellar theory through the analysis of radial and non-radial stellar oscillations. Here, the presence of such oscillations in more than 300 giant stars is reported, with mode lifetimes of some of the giants in the order of a month.

Loss of the structural microtubule-associated protein 6 (MAP6) leads to neuronal differentiation defects that are independent of MAP6’s microtubule-binding properties. Here the authors establish a functional link between MAP6 and Semaphorin 3E signalling for proper formation of the fornix of the brain.