Search

Knock-in mice conditionally or constitutively expressing Cas12 variants enable a wide range of ex vivo and in vivo applications requiring multiplexed genome engineering.

In this work, authors utilise a prefractionation natural product extract screening platform, which uncovers coniotins, lipopeptaibiotics with broad antifungal activity and a mechanism of action that targets fungal cell wall β-glucan.

The genetic basis of grapevine thermotolerance remains poorly understood. Here, the authors identify WRKY transcription factor TTC4 as a positive regulator of thermotolerance and reveal its possible upstream repressor SPL13 and downstream target genes HSP18.1 and APX3.

Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combination enhances the resolution, throughput and reproducibility for data-independent acquisition-based mass spectrometry analysis.

Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

The approval of first line immune checkpoint blockade (ICB) has improved outcomes for patients with metastatic non-small cell lung cancer (mNSCLC), however, whether patients would benefit more from ICB alone or alongside chemotherapy is unclear. Here, the authors develop a machine-learning based approach to help guide individual treatment selection patients with mNSCLC.

To date, experimental induction of hair cell regeneration in mammals leads to immature and poorly differentiated hair cells. Here the authors show that the transcription factor prdm1a plays a crucial role in specifying sensory hair cell types with loss of prdm1a in zebrafish leading to misspecification of hair cells in the sensory lateral line system into ear hair cells.

An optically addressable fluorescent-protein spin qubit is realized using enhanced yellow fluorescent protein; the qubit can be coherently controlled at liquid-nitrogen temperatures and the spin detected at room temperature in cells.

Effective treatments are lacking for sepsis-related heart failure, which involves severe impairment of heart cell metabolism. Here, the authors show that blocking the protein MacroD1 preserves cellular energy production and protects against heart failure in mouse models of sepsis.

The development of moderate magnetic hyperthermia (MH) for tumor ablation while concurrently restoring bone homeostasis shows potential for osteosarcoma (OS) therapy. Here this group combines magnetothermal nanoparticles with MH temperature-controlled borosilicate bioactive glass achieving OS cell impair while activating TNF signaling pathway for therapeutic purpose.

Xiang et al. use patient samples and mouse models of osteoarthritis to examine the link with muscle atrophy. They identify that, in osteoarthritis, senescent macrophages promote ferroptosis in skeletal muscle cells, and they show the therapeutic value of targeting this pathological mechanism.

Breast cancer cells interact with neighbouring adipocytes, but the mechanisms are not fully understood. Here, the authors show that triple-negative breast cancer (TNBC) cells transfer cAMP through gap junctions, activating lipolysis in tumour-associated adipocytes to promote TNBC growth.

CD4⁺ T cells promote immunity to tuberculosis infection via macrophages. Here the authors show upregulation of SLAMF1/CD150 on infected macrophages after interaction with CD4⁺ T cells and that the presence of SLAMF1 promotes ROS production by macrophages and the absence of Slamf1 in macrophages results in higher mycobacterial loads in infected mice.

Researchers show how a viral collagen glucosyltransferase works by solving its 3D structure, revealing dimerization-driven activity and its surprising ability to make the prebiotic sugar kojibiose, offering clues for drug and prebiotic development.

Simulating molecular adsorption on surfaces presents considerable challenges, as computational methods typically suffer from either insufficient accuracy or prohibitive computational costs. Now, with an open-source multilevel embedding approach, adsorption processes on the surfaces of ionic materials can be modelled routinely with an accuracy comparable to that of experiments.

Here authors demonstrate how a 2D hybrid perovskite melts and forms glass, uncovering atomic-scale structural and dynamic evolution across the crystal–liquid–glass transition. Local structural motifs are retained, advancing understanding of amorphous hybrid materials.

Analyses of consummatory reproductive behaviours in male mice uncover a brain mechanism whereby an internal state can attribute a social quality to a generic touch to initiate purposeful reproductive actions.

In this study, Yang et al. compile a global dataset to uncover the degree to which plants coordinate root and seed traits. They report a global positive correlation between root diameter and seed size, driven by dual roles of arbuscular mycorrhiza in phosphorus uptake and pathogen defence.

Cooling in natural systems typically requires external contact with a cold reservoir, with the final temperature determined by the environment, as dictated by thermodynamics. The authors demonstrate a spontaneous, selfsustained frictional cooling effect driven by the interplay of activity, dry friction, and crowding.

Using intracranial EEG recordings in epilepsy patients, Pacheco-Estefan et al. describe changes in the neural representations of cues and contexts during fear and extinction learning in the human brain.

Artificial intelligence-based detection of gastric cancer at different stages from noncontrast computed tomography is suggested to be feasible in a retrospective analysis of large and diverse cohorts, including real-world populations in opportunistic and targeted screening scenarios.

McGavern and colleagues identified a CD8⁺ T cell population that expresses GZMK in ʽtau-richʼ brains, where it may play a protective role against neurodegeneration.

This pilot trial showed that perioperative treatment with the isocitrate dehydrogenase (IDH) inhibitor safusidenib of patients with low-grade IDH-mutant glioma, with craniotomy and lumbar puncture before and after treatment, is feasible and safe and enabled in-depth translational investigation of safusidenib treatment-induced changes in the tumor, including electrophysiological effects.

Targeting CD8⁺ T cell exhaustion is a strategy to enhance immune checkpoint inhibition and to fight cancer. Here the authors show a NRF2-dependent role for the prostaglandin I₂ receptor PTGIR in controlling T cell exhaustion.

The PrimeGen framework is a multi-agent large language model system used to navigate intricate primer design workflows for amplicon sequencing.

The efficacy of CAR-T cells against solid tumors is still limited by immunosuppressive factors. Here, the authors show that engineering of CAR T cells with A1R enhances their efficacy against solid tumors in vitro and in vivo.

Liu and colleagues examine how severe acute respiratory syndrome coronavirus 2 variants evade CD8⁺ T cell epitope recognition.

Some cancer cells exhibit high loads of reactive iron in lysosomes, and this feature is exploited by using fentomycin-1, a newly developed small molecule, to induce ferroptosis.

This study identified prevalent giant extrachromosomal elements from the human oral microbiome. The genetic and ecological analysis provides insight on the potential contribution of this element in adaptation against multiple oral environmental stressors.

Direct CO₂ conversion into high-value organic products faces significant selectivity challenges. This work presents a FeCo carbide-based tandem catalyst enabling high-efficiency, stable production of C2+ alcohols.

This multiomic study, including single-nucleus DNA methylation and chromatin conformation matched with single-nuclei RNA sequencing, provides insights into the epigenomic landscape of human subcutaneous adipose tissue.

TopoVelo infers cell differentiation stages and rate parameters of gene expression using spatially coupled differential equations.

The function of histone lysine β-hydroxybutyrylation (Kbhb) is unclear in plants. Here, the authors show that H3K9bhb is enriched at transcription start sites in rice and regulates gene expression during immune responses.

Timely identification and treatment of cerebral lesions in high-risk neonates are crucial. Here, the authors present a deep learning system that extracts multiple standard views from neonatal cranial ultrasound videos and diagnoses severe cerebral lesions with high accuracy, outperforming junior radiologists.

Semiconductor quantum dots that simultaneously support blue and yellow emission offer a route to realizing efficient white light-emitting diodes.

Dual-scale chemical ordering in CoNiV-based alloys improves the synergy of strength and ductility at cryogenic temperatures, providing an approach for obtaining high-performance metallic materials for cryogenic applications.

This large meta-analysis of 11 GWAS identified 7 genetic loci associated with strabismus, esotropia and exotropia. Mendelian randomisation provided genetic evidence of the causal link between maternal smoking to increased strabismus risk in offspring.

The LHCb experiment at CERN has observed significant asymmetries between the decay rates of the beauty baryon and its CP-conjugated antibaryon, thus demonstrating CP violation in baryon decays.

The authors analyze rare coding variants in 1990 individuals with congenital kidney anomalies, finding diagnostic variants in 14.1% of cases. They identify two new causal genes, ARID3A and NR6A1, along with 38 candidate genes, providing evidence for shared genetics with other developmental disorders.

Nonlinear transport effects arising from the quantum metric have been reported in topological magnets at low temperatures. Here, the authors demonstrate a second-harmonic transport response in TbMn₆Sn₆ at room temperature, attributed to the quantum metric and controllable via an applied magnetic field.