Determining relevant targets of promiscuous kinase inhibitors has proven difficult. A combination of two mass spectrometry–based proteomic approaches, RNAi depletion and rescue studies with drug-resistant mutants now reveal that SRC, FYN and EGFR are functionally important targets of dasatinib in lung cancer cells.
- Jiannong Li
- Uwe Rix
- Eric B Haura
