Figure 2
ARR2PBneo cells selectively cultured for 4 weeks in epithelial cell culture medium containing 400 μg/ml G418 and ARR2PBneo × LPB-Tag prostate cell culture can be selected by G418. (a) MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay of NeoTag1 cells at third passage. Cells treated with different concentration of G418 (0, 100, 200, 300, 400, 600 μg/ml) for 5 days. NeoTag cell shows G418 resistance compared with 12T–7f cells. (b) MTT assay of NeoTag1 cells after 1 month G418 selection shows strong G418 resistance. Cells treated with different concentration of G418 (0, 100, 200, 300, 400, 500, 600, 800 μg/ml) for 5 days. The results represent the average±s.d. of three independent experiments; each was performed in triplicate.
