Figure 6
Immunohistochemistry of tissue recombinants composed of rat UGM with early passage NeoTag epithelial cells 4 weeks post grafting to an intact male athymic mouse host (NeoTag1, (a, d, g) NeoTag2, (b, e, h) and NeoTag3 (c, f, i)). Immunohistochemistry was used to determine AR, CK8, and T-antigen expression in the tissue recombination. (a) AR immunostaining with positive strong diffuse nuclear pattern. Majority of histology is PIN with foci adenocarcinoma. (b) Infiltrating adenocarcinoma with positive nuclear staining for AR. (c) Focal positive nuclear staining for AR with prominent nuclear pleomorphism consistent with adenocarcinoma. (d–f) Positive strong diffuse cytoplasmic and luminal staining for cytokeratin 8. (g–i) Positive strong diffuse nuclear staining throughout the epithelium for T-antigen. Bars, 50 μm.
