Figure 9
BrdU staining of NeoTag1 and NeoTag2 grafts before and after castration. (a) BrdU staining of proliferating cells in NeoTag1 and 2 grafts including intact grafts, after 2 days castration and after 2 weeks castration. Abundant nuclear staining throughout the tumor with expanded gland profiles was seen in the intact grafts of NeoTag1 and NeoTag2. In the grafts after 2 days castration, numbers of positive cells is decreased compared to the intact grafts, with NeoTag 1 and 2 showing focal nuclear positivity. NeoTag 1 and 2 show rare nuclear positivity after 2 weeks castration. (b) NeoTag1 graft cell proliferation index was determined after counting the number of total labeled cells divided by the total cell number counted. The asterisks indicate significance differences between intact and castrated mice (***P<0.0001) by ANOVA F-test. (c) NeoTag2 graft cell proliferation index was determined after counting the number of total labeled cells divided by the total cell number counted. The asterisks indicate significance differences between intact and castrated mice (***P<0.001) by ANOVA F-test. Bars, 50 μm.
