Table 1 Oligonucleotides for RT-PCR used in the study
Primer | Primer sequence (5′–3′) | Tm (°C) | Product size (bp) |
|---|---|---|---|
PtenF | TCCCAGACATGACAGCCATCATCA | 59.8 | 300 |
PtenR | GCTGTGGTGGGTTATGGTCTTCAA | 59.0 | |
MycF | GGGACAGTGTTCTCTGCCTCT | 58.4 | 200 |
MycR | CTCTTCCTCGTCGCAGATGAAATAGG | 58.5 | |
HepsinF | TCTCATTGTGGGTACCCTGCTGTT | 60.0 | 375 |
HepsinR | AATCGGCCTCTAGGACAGTCACAT | 59.3 | |
β-CateninF | TGGGACTCTGCACAACCTTTCTCA | 60.0 | 313 |
β-CateninR | ACCACTGGCCAGAATGATGAGCTT | 60.5 | |
ARF | AACCAACCAGATTCCTTTGCTGCC | 60.0 | 606 |
ARR | TTCAGGAAAGTCCACGCTCACCAT | 60.3 | |
Nkx3.1F | CTGAACCCGAGTCTGATGCACATT | 59.0 | 217 |
Nkx3.1R | AGTTTGAGGTTCTTGGCCAGGTGA | 60.3 | |
Pim-1F | GCGGCGAAATCAAACTCATCGACT | 59.6 | 314 |
Pim-1R | TTCTTCAAAGGAGGGCCGATCTGA | 59.7 | |
PSCAF | TTTCTCCTGCTGGCCACCTACTTA | 59.5 | 247 |
PSCAR | AGCAGCACGTGATGTTCTTCTTGC | 59.9 | |
ClusterinF | TGAGCTCCAAGAACTGTCCACTCA | 59.5 | 305 |
ClusterinR | TCCTGCAGACACGTGCATAGAACT | 59.9 | |
GAPDHF | ACCACAGTCCATGCCATCAC | 58.6 | 452 |
GAPDHR | TCCACCACCCTGTTGCTGTA | 59.7 |
