Table 1 Enhancement of Mitogenic Activity of Human VSMC by NHDL3 and APHDL3 Unhydrolyzed and Hydrolyzed by gr IIA sPLA2a

From: Mitogenic Effect of Lipoproteins on Human Vascular Smooth Muscle Cells: The Impact of Hydrolysis by gr II A Phospholipase A2

Agents

Ab

Bc

Control

12577 ± 2539+

8140 ± 1273+

NHDL3

24201 ± 1250

18674 ± 1490

NHDL3 + sPLA2

30701 ± 855

41285 ± 791

APHDL3

36743 ± 1113

18925 ± 1064

APHDL + sPLA2

41240 ± 4588

39784 ± 1955

LDL

23331 ± 2099

33891 ± 3102

LDL + sPLA2

43924 ± 3520

39784 ± 1995

  1. VSMC, vascular smooth muscle cells; NDHL, normal high-density lipoprotein; APHDL3, acute phase HDL3, LDL, low-density lipoprotein.
  2. aExample of 6 experiments with unhydrolyzed NHDL3 and APHDL3, and 12 experiments with sPLA2-hydrolyzed lipoproteins, each in triplicate.
  3. bConcentration of NHDL3, APHDL3, and LDL 40 μg/ml; sPLA2 100 ng/ml. Preincubation time, 4 hours.
  4. cConcentration of LDL and NHDL3, 50 μg/ml; APHDL3, 25 μg/ml; sPLA2, 100 ng/ml. Preincubation time, 24 hours.
  5. 3H Thymidine incorporation (cpm/well). Each experiment in triplicate. Means ± sd.
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