Abstract
Exposure of phosphatidylserine on the outer leaflet of the cytoplasmic membrane is an early event during apoptotic cell death and serves as a recognition signal for phagocytes. Usually the clearance of apoptotic cells does not initiate inflammation or immune response. We investigated the immune response in Balb/c mice towards apoptotic human T-cells. Animals injected with apoptotic cells showed significantly reduced humoral immune responses, especially Th1-dependent IgG2a titres, compared to controls immunised with viable cells. However, treatment of apoptotic cells with annexin V (AxV) significantly increased the humoral immune response. AxV binds with high affinity to anionic phospholipids and as a result interferes with the phosphatidylserine recognition by phagocytes. Our results indicate that AxV treatment may be used to increase the efficiency of apoptotic cell-based vaccines, e.g. some tumour vaccines. Cell Death and Differentiation (2000) 7, 911–915
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Abbreviations
- AxV:
-
annexin-V
- a-huL:
-
anti-human-T-lymphocyte
- APC:
-
antigen presenting cells
- FA:
-
incomplete Freund's adjuvant
- PHA:
-
phytohemagglutinin
- PS:
-
phosphatidylserine
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Acknowledgements
We thank Dr. Michael May (Yale University, New Haven, CT, USA) for critical reading of the manuscript. Supported by BMFT KBF 01 GB9403 and Bayerische Forschungsstiftung FORGEN.
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Stach, C., Turnay, X., Voll, R. et al. Treatment with annexin V increases immunogenicity of apoptotic human T-cells in Balb/c mice. Cell Death Differ 7, 911–915 (2000). https://doi.org/10.1038/sj.cdd.4400715
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DOI: https://doi.org/10.1038/sj.cdd.4400715
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