Abstract
Mammary cell apoptosis and proliferation were assessed after injection of Escherichia coli into the left mammary quarters of six cows. Bacteriological analysis of foremilk samples revealed coliform infection in the injected quarters of four cows. Milk somatic cell counts increased in these quarters and peaked at 24 h after bacterial injection. Body temperature also increased, peaking at 12 h postinjection. The number of apoptotic cells was significantly higher in the mastitic tissue than in the uninfected control. Expression of Bax and interleukin-1β converting enzyme increased in the mastitic tissue at 24 h and 72 h postinfection, whereas Bcl-2 expression decreased at 24 h but did not differ significantly from the control at 72 h postinfection. Induction of matrix metalloproteinase-9, stromelysin-1 and urokinase-type plasminogen activator was also observed in the mastitic tissue. Moreover, cell proliferation increased in the infected tissue. These results demonstrate that Escherichia coli-induced mastitis promotes apoptosis and cell proliferation. Cell Death and Differentiation (2001) 8, 808–816
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Abbreviations
- CFU:
-
colony forming units
- E. coli :
-
Escherichia coli
- ECM:
-
extracellular matrix, ICE, interleukin-1β converting enzyme
- IL-1:
-
interleukin-1
- LPS:
-
lipopolysaccharide
- MMP:
-
matrix metalloproteinase
- PA:
-
plasminogen activator
- PAI:
-
plasminogen activator inhibitor
- RT-PCR:
-
reverse-transcriptase polymerase chain reaction
- S. agalactiae :
-
Streptococcus agalactiae
- S. aureus :
-
Staphylococcus aureus
- SCC:
-
somatic cell counts
- SL:
-
stromelysin
- SV40 Tag:
-
Simian virus 40 T antigen
- TIMP:
-
tissue inhibitor of metalloproteinase
- TNF α:
-
tumor necrosis factor α
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Acknowledgements
This work was partially supported by a grant (155423-98) from Natural Sciences and Engineering Research Council of Canada to X Zhao.
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Long, E., Capuco, A., Wood, D. et al. Escherichia coli induces apoptosis and proliferation of mammary cells. Cell Death Differ 8, 808–816 (2001). https://doi.org/10.1038/sj.cdd.4400878
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DOI: https://doi.org/10.1038/sj.cdd.4400878
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