Abstract
By high density oligonucleotide microarrays we have studied the expression profile of proliferating and VD treated HL60 cells and the molecular phenotype of VD monocytes and that of CD14+ peripheral monocytes has been compared. The results indicate that important changes in functional categories of the differentially expressed genes underlie the differentiation transition from myeloblasts to monocytes. This differential gene expression pattern leads to an increased expression of mRNAs involved in surface and external activities since many of the VD induced genes belong to ligand binding, receptors, cell surface antigens, defense/immunity and adhesion molecules functional categories. The results also indicate that the molecular phenotypes of monocytes and VD induced cells diverge for a small but significant set of defense related genes. Particularly, class II MHC genes are not expressed in these cells. Furthermore, the high levels of expression of these genes induced by serum treatment of monocytes are decreased by VD.
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Abbreviations
- AML:
-
acute myeloid leukemia
- ATRA:
-
all-trans retinoic acid
- avg. diff.:
-
average difference
- DMSO:
-
dimethylsulphoxide
- DMT:
-
data mining tool
- FAB:
-
French-American-British Classifiaction
- FBS:
-
fetal bovine serum
- GO:
-
gene ontology
- IMDM:
-
Iscove's modified Dulbecco's medium
- MGG:
-
May-Grünwald and Giemsa
- MAS 4.0:
-
microarray analysis suite 4.0
- MHC:
-
major histocompatibility complex
- PBMC:
-
peripheral blood mononuclear cells
- RT–PCR:
-
reverse transcription-polymerase chain reaction
- VD:
-
1,α 25 dihydroxivitamin D3
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Acknowledgements
The authors gratefully acknowledge the generous support of the ‘Cassa di Risparmio Foundation of Modena’ for the acquisition of the ‘Affymetrix System’. This work was supported by AIL (Italian Association against Leukemia), AIRC (Italian Association for Cancer Research), CIB (Italian Consortium for Biotechnology) and MURST-2000.
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Tagliafico, E., Tenedini, E., Bergamaschi, A. et al. Gene expression profile of Vitamin D3 treated HL60 cells shows an incomplete molecular phenotypic conversion to monocytes. Cell Death Differ 9, 1185–1195 (2002). https://doi.org/10.1038/sj.cdd.4401104
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DOI: https://doi.org/10.1038/sj.cdd.4401104
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