Abstract
Apoptosis is often accompanied by degradation of chromosomal DNA. CAD, caspase-activated DNase, was identified in 1998 as a DNase that is responsible for this process. In the last several years, mice deficient in the CAD system have been generated. Studies with these mice indicated that apoptotic DNA degradation occurs in two different systems. In one, the DNA fragmentation is carried out by CAD in the dying cells and in the other, by lysosomal DNase II after the dying cells are phagocytosed. Several other endonucleases have also been suggested as candidate effectors for the apoptotic degradation of chromosomal DNA. In this review, we will discuss the mechanism and role of DNA degradation during apoptosis.
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Abbreviations
- CAD:
-
caspase-activated DNase
- ICAD:
-
inhibitor of CAD
- DFF:
-
DNA fragmentation factor
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Acknowledgements
The work in our laboratory was supported in part by Grants-in-aid from the Ministry of Education, Science, Sports, and Culture in Japan. KK and NM were supported by a Research fellowship of the Japan Society for the Promotion of Science. We thank Drs. L Migletta and G Gray of Clarity Editing for the careful editing of the manuscript.
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Nagata, S., Nagase, H., Kawane, K. et al. Degradation of chromosomal DNA during apoptosis. Cell Death Differ 10, 108–116 (2003). https://doi.org/10.1038/sj.cdd.4401161
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