Abstract
C-myc gene is a member of the myc family of proto-oncogenes involved in proliferation, differentiation, and apoptosis. Overexpression of c-myc in fibroblasts causes apoptosis under low serum conditions in a process that requires the interaction of CD95 and CD95L on the surface. We have previously reported an in vivo conditional model to inactivate the c-myc gene in B lymphocytes. Here, we show that c-Myc-deficient primary B lymphocytes are resistant to different apoptotic stimuli. Nonactivated c-Myc-deficient B cells are resistant to spontaneous cell death. Upon activation, c-Myc-deficient B lymphocytes express normal surface levels of activation markers, and show resistance to staurosporine and CD95-induced cell death.
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Abbreviations
- fl:
-
floxed allele
- Δ:
-
deleted allele
- AICD:
-
activation-induced cell death
- bHLHZip:
-
basic region/helix–loop–helix/leucine zipper
- GEP:
-
green fluorescence protein
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Acknowledgements
We thank CMA's lab members for their input, and X Mao and S Orkin for the EGFP reporter mice. IMA thanks FW Alt for his input and support. We are very grateful to all the staff of the animal facilities at CNB-CSIC for their help. IMA is supported by a Ramón y Cajal (RyC) fellowship, and EB by a predoc fellowship from the Spanish Ministry of Science and Technology. This work was supported by grants from the Ministry of Science and Technology and EU. The DIO was founded and is supported by the Spanish Council for Scientific Research (CSIC) and by PFIZER.
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de Alborán, I., Baena, E. & Martinez-A, C. c-Myc-deficient B lymphocytes are resistant to spontaneous and induced cell death. Cell Death Differ 11, 61–68 (2004). https://doi.org/10.1038/sj.cdd.4401319
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DOI: https://doi.org/10.1038/sj.cdd.4401319
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