Abstract
We describe here the identification and initial characterization of a novel human gene termed IKIP (I kappa B kinase interacting protein) that is located on chromosome 12 in close proximity to APAF1 (apoptotic protease-activating factor-1). IKIP and APAF1 share a common 488 bp promoter from which the two genes are transcribed in opposite directions. Three IKIP transcripts are generated by differential splicing and alternative exon usage that do not show significant homology to other genes in the databases. Similar to APAF1, expression of IKIP is enhanced by X-irradiation, and both genes are dependent on p53. Moreover, IKIP promotes apoptosis when transfected into endothelial cells. We conclude that IKIP is a novel p53 target gene with proapoptotic function.
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Abbreviations
- APAF1:
-
apoptotic protease-activating factor-1
- ECFP:
-
enhanced cyan fluorescent protein
- EGFP:
-
enhanced green fluorescent protein
- EYFP:
-
enhanced yellow fluorescent protein
- ER:
-
endoplasmic reticulum
- HUVECs:
-
human umbilical vein endothelial cells
- IKIP:
-
I kappa B kinase interacting protein
- LPS:
-
bacterial lipopolysaccharide
- RT-PCR:
-
real-time PCR
- TN:
-
tunicamycin
- TNF:
-
tumor necrosis factor
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Acknowledgements
We thank Peter Gold for excellent technical assistance. This work was supported by a grant from the Austrian Science Foundation (FWF) within the SFB ‘Microvascular Injury and repair’, project 05-12, to RdM.
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Hofer-Warbinek, R., Schmid, J., Mayer, H. et al. A highly conserved proapoptotic gene, IKIP, located next to the APAF1 gene locus, is regulated by p53. Cell Death Differ 11, 1317–1325 (2004). https://doi.org/10.1038/sj.cdd.4401502
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DOI: https://doi.org/10.1038/sj.cdd.4401502
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