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Quantitative real-time reverse transcription: polymerase chain reaction of prostate-specific antigen (PSA) for detection of circulating prostatic cells in patients with clinically localized prostate cancer

Prostate Cancer and Prostatic Diseases volume 8, pages 248–252 (2005)Cite this article

Abstract

Objective: To evaluate the clinical utility of using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify prostate-specific antigen (PSA) mRNA in peripheral blood samples from patients with prostate cancer as a predictor of extraprostatic extension of the disease and to assess any correlations with known predictive markers of this condition.

Methods: Immediately before radical prostatectomy, peripheral blood samples were taken from 42 men with clinically localized prostate cancer and analysed for PSA and 18S ribosomal (endogenous control) genes using real-time RT-PCR (with gene expression assays and the comparative CT–cycle threshold—method for quantifing). A total of 30 healthy male blood donors aged <50 y was taken as a control group.The relationships between PSA mRNA values, pathological and clinical features were analysed. PSA mRNA value, PSA level and biopsy Gleason score were then compared as predictors of extraprostatic extension.

Results: PSA gene expresion was 3.73 times significantly higher in patients with clinically localized prostate cancer than in healthy men (P<0.05). There was no relationship between PSA real-time RT-PCR values and pathological stage pT2 or pT3 (P=0.5), and no association between PSA mRNA value and serum PSA level (P=0.9) or the Gleason score of the preoperative biopsy (P=0.9).

Conclusion: There was no significant advantage in using the real-time RT-PCR assay of PSA mRNA before surgery to stage prostate cancer and to discriminate between organ-confined and extraprostatic extension.

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Acknowledgements

We express our appreciation to the staff of the Department of Anaesthesiology in the Hospital Universitario de Getafe, especially Yolanda Cabrero and María Luisa Mariscal, and also to María Dolores Menor (haematologist). Partly supported by grant from the ‘Fundación para la Investigación en Urología’ FIU 2002 (L LL).

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Authors and Affiliations

  1. Department of Urology, Hospital Universitario de Getafe, Madrid, Spain

    L Llanes, A Ferruelo, M Luján, C Pascual, J M García-Mediero & A Berenguer

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  1. L Llanes
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  2. A Ferruelo
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  3. M Luján
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  4. C Pascual
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  5. J M García-Mediero
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  6. A Berenguer
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Correspondence to A Berenguer.

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Llanes, L., Ferruelo, A., Luján, M. et al. Quantitative real-time reverse transcription: polymerase chain reaction of prostate-specific antigen (PSA) for detection of circulating prostatic cells in patients with clinically localized prostate cancer. Prostate Cancer Prostatic Dis 8, 248–252 (2005). https://doi.org/10.1038/sj.pcan.4500801

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