Fig. 1
From: A stable triple Wolbachia infection in Drosophila with nearly additive incompatibility effects
Heteroduplex assay. Lanes 1 and 12, ‘1 kb’ ladder (Gibco). Lanes 2–4 contain products from single Wolbachia strains: wHa (DSH) in lane 2; wNo (R3A) in lane 3 and wRi (DSR) in lane 4. Lanes 5–9 each contain products from two strains, either from double-infected flies or obtained as mixtures of PCR products: wHa + wNo (DSH + R3A in lane 5 and Nou in lane 6); wHa + wRi (DSH + DSR in lane 7 and M4 in lane 8); wNo + wRi (R3A + DSR) in lane 9. Products of the three Wolbachia strains are shown in lanes 10 (DSH + R3A + DSR) and 11 (HNR2). The wHa and wRi products form an obvious pair of heteroduplexes in lanes 7, 8, 10 and 11. The wNo product forms heteroduplexes with either wHa or wRi in lanes 4, 5, 9, 10 and 11. As the wHa and wRi wsp sequences are more similar to each other than to wNo (Zhou et al., 1998), the heteroduplexes involving wNo products have lower electrophoretic mobility and are formed in lower amounts than the wHa + wRi heteroduplexes.
