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Pre-Clinical Studies

Hexaminolevulinate-mediated photodynamic purging of marrow grafts with murine breast carcinoma

Bone Marrow Transplantation volume 46, pages 1118–1127 (2011)Cite this article

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Abstract

Photodynamic therapy (PDT) with porphyrin precursors is an established therapy for certain tumors. This study aimed to explore the use of hexaminolevulinate (HAL), a porphyrin precursor, for photodynamic purging of BM grafts contaminated with cells of the 4T1 breast carcinoma cell line. The optimal PDT dose was not effective in eradicating 4T1 cells when the tumor cells were mixed with murine marrow cells in vitro. However, the number of pulmonary metastases was reduced, and the survival of experimental animals was prolonged substantially when they were subjected to TBI followed by transplantation of syngeneic BM containing metastasized 4T1 cells that had been treated ex vivo by HAL-PDT. Despite the failure of in vitro experiments, HAL-based photodynamic purging could be a useful modality for treating animals bearing an experimental breast carcinoma.

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Acknowledgements

The authors are grateful to PhotoCure ASA for providing HAL, Professor Vivi Ann Flørenes and Dr Anne Tierens for valuable discussions; and Anne Katrine Rosnes, Tove Anita Slyngstad, Marianne Sand Dyrhaug, Renathe Fjelldal, Marit Helgerud and Vladimir Iani for technical assistance. Beata Čunderlíková is grateful for grant supports from The Research Council of Norway (project 172818) and The Norwegian Radium Hospital Research Foundation (project 0609).

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Authors and Affiliations

  1. Department of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

    B Čunderlíková, V Vasovič, E Borgen, J M Nesland & Q Peng

  2. International Laser Centre, Bratislava, Slovakia

    B Čunderlíková

  3. Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA

    F Sieber

  4. Department of Medical Physics, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

    T Furre

  5. Faculty Division, Norwegian Radium Hospital, Medical faculty, University of Oslo, Oslo, Norway

    J M Nesland

  6. Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, China

    Q Peng

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  1. B Čunderlíková
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Correspondence to Q Peng.

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Čunderlíková, B., Vasovič, V., Sieber, F. et al. Hexaminolevulinate-mediated photodynamic purging of marrow grafts with murine breast carcinoma. Bone Marrow Transplant 46, 1118–1127 (2011). https://doi.org/10.1038/bmt.2010.277

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