Figure 1
From: The circadian mutation PER2S662G is linked to cell cycle progression and tumorigenesis
The PER2S662 mutation leads to decreased levels of apoptosis. (a) Wild-type, Per2−/−, PER2S662G:Per2−/− and PER2S662D:Per2−/− mice were irradiated with 6 Gy X-rays at ZT 0 (0800 hours) and ZT 9 (1700 hours). Spleen cells were isolated 6 h after irradiation, stained with propidium iodide (PI) and analyzed by flow cytometry. One representative experiment is shown. Ap, apoptotic percentage. (b) Percentages of apoptotic cells measured by flow cytometry are shown in the histograms. Experiments were repeated at least three times with consistent results and at least two mice were used per group for each time (N=3 for each genotype; ***P<0.001; one-way ANOVA followed by Dunnett's test)
