Figure 7
From: The circadian mutation PER2S662G is linked to cell cycle progression and tumorigenesis
The relative phases of cell cycle genes are shifted by PER2 mutations. Wild-type and different PER2 mutant MEFs were synchronized by a dexamethasone treatment, and RNA was extracted at 3 h intervals stating 10 h after the shock. p21, Cyclin D1 and Nr1d1 expression analysis were done by q-PCR. Experiments were repeated independently at least three times with consistent results. Plotted values are the mean values±S.D. Cosinor analysis was performed on mRNA profiles using SPSS 17.0. The amplitude and acrophase obtained from cosinor analysis were further analyzed by Students t-test. The null hypothesis was rejected at P<0.05
