Figure 1

CD300f expression profile in immune cell populations. (a) Indicated immune cells were sorted from splenocytes or differentiated from bone marrow cells for cDNA preparation and CD300f mRNA expression levels were determined. The graph shown is representative of two independent experiments. (b) The cell surface CD300f expression was analyzed by flow cytometry on the following populations of cells: cDC, conventional DC (CD11c^hiB220⁻), pDC, plasmacytoid DC (CD11c^loB220⁺PDCA-1⁺), Mon/Mac, monocyte or macrophages (CD11b^hiCD11c⁻Ly6G⁻SSC^lo), FO B, follicular B cells (B220⁺CD21^loCD23⁺), MZ B, marginal zone B cells (B220⁺CD21^hiCD23⁻), TR B, transitional B cells (B220⁺CD21^lo/−CD23⁻), BMDC^−/−, bone marrow-derived DC from Cd300f^−/− mice, BMM^−/−, bone marrow-derived macrophages from Cd300f^−/− mice, PB, plasmablast cells (B220⁺CD138⁺), PC, plasma cells (B220^lo/−CD138⁺). The data is represented as the relative expression of CD300f on cells from Cd300f^+/+ mice normalized to those from Cd300f^−/− mice. The line indicates the level of background staining on cells from Cd300f^−/− mice. The graph shows the mean+S.E.M. from three separate analyses. Asterisks indicate statistical significance (*P<0.05, ***P<0.005). (c) CD300f expression on the cell surface of BMM and BMDC isolated from Cd300f^+/+ or Cd300f^−/− mice. The graph shown is representative of two experiments