Figure 1
From: XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair
DNA-PKcs co-purifies with all known core NHEJ components. (a) Nuclear extracts (lanes 1 and 2) or eluates of purified anti-FLAG IPs from either HEK293 control (lane 5) or FLAG-DNA-PKcs-expressing cells (lane 6) were resolved by SDS-PAGE and visualised by silver staining. (b) As described in a, except that core NHEJ proteins were detected by immunoblotting. **Position of unspecific protein band. (c) Eluates of purified anti-FLAG IPs from either untreated (lanes 1 and 2) or irradiated (10 Gy, lanes 3 and 4) HEK293 control, or untreated (lanes 5 and 6) or irradiated (10 Gy, lanes 7 and 8) FLAG-DNA-PKcs-expressing cells were resolved by SDS-PAGE and visualised by silver staining. Nuclear extracts were incubated in the absence (lanes 1, 3, 5 and 7) or presence (lanes 2, 4, 6 or 8) of EtBr (0.2 mg/ml) for 30 min before addition of anti-FLAG M2 beads. (d) As described in c, except that core NHEJ proteins were detected by immunoblotting. **Position of unspecific protein band
