Figure 2
From: XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair
An uncharacterised protein c9orf142 associates with DNA-PKcs-containing NHEJ complexes. (a) Table showing 10 most abundant proteins identified in anti-FLAG IPs from FLAG-DNA-PKcs but not HEK293 control cells (average protein abundance from two independent experiments was used in creation of the ranking list). C9orf142 is shown in red (proteins are named based upon UniProt nomenclature. CI142_HUMAN indicates c9orf142/XLS). (b) Sequence coverage for c9orf142/XLS. Amino acids, which were identified within peptides detected by LC-MS/MS, are marked in red. The total % sequence coverage was 49%. (c) Nuclear extracts (lanes 1 and 2) or eluates of isolated anti-FLAG IPs from nuclear extracts from either HEK293 control (lanes 3–6) or FLAG-DNA-PKcs expressing cells (lanes 7–10), which had been incubated with increasing concentrations of EtBr before addition of anti-FLAG beads were resolved by SDS-PAGE. Ku70, Ku86 and c9orf142 were detected by immunoblotting. (d) Identification of core NHEJ proteins from FLAG-DNA-PKcs IPs by LC-MS/MS mass spectrometry. Results shown in each column are the average quantities (fmol) of the indicated protein detected from duplicate injections from a total of four independent experiments
