Figure 3
From: XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair
Computer modelling of c9orf142/XLS protein predicts a structure similar to XRCC4. (a) 3D structural model for full-length human c9orf142/XLS generated using the Phyre2 server, which predicted with 95% and 85% confidence overlapping structural features between amino acids 5–160 of human c9orf142/XLS with human XRCC4 and XLF (crystal structures), respectively. Although XRCC4 and XLF typically form dimers, the figure shows monomers for all proteins to highlight their structural similarities. ‘N’ and ‘C’ indicate positions of the N- and C terminus of XLS, respectively. (b) Schematic figure showing the predicted structural and function domains for c9orf142/XLS. These are a N-terminal head domain, a middle coiled-coil domain and a C-terminal nuclear localisation signal (NLS). (c) Primary sequence alignment of c9orf142/XLS from human, chimpanzee, mouse, rat, zebrafish, Xenopus and starlet sea anemone (coral) species. Identical amino acids are indicated as shaded black areas. (d) Predicted 3D structural model generated using Phrye2 server for full-length starlet sea anemone c9orf142/XLS, which shares ~20% sequence identity with human XLS (not shown)
