Figure 1

NF-κB-dependent downregulation of Tipe2 in T cells and myeloid cells. (a) Murine naive CD4+ T cells were either untreated or treated with plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (1 μg/ml) for the indicated times, and Tipe2 mRNA levels were determined by quantitative PCR. (b) Murine naive CD4+ T cells were either untreated or treated with plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (1 μg/ml) for 5 h, Tipe2 protein levels were determined by western blot analysis. (c) Murine naive CD4+ T cells were treated as in (a) for 4 h in the absence or presence of NF-κB inhibitor Bay 11-7082. Tipe2 mRNA levels were determined by quantitative PCR. *P<0.02. The results are representative of three independent experiments