Abstract
Young embryos of rice (Oryza sativa L. subsp, japonica var. Guo-xiang No. I) were cultured on MS agar medium(2,4-D 2 mg/l). Calli were formed and subcultured on N6 agar medium (2,4-D 2 mg/l). After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium(2,4-D 1 mg/l, 6-BA 0.2 mg/l) 1* with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.
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Notes
2,4-D-2, 4-dichlorophenoxyacetic acid.6- BA - 6- benzyladenine.
YE-Yeast extract.CM-Cocoanut milk.
MES-2(N-morpholino) ethane sulphonic acid
ZT-Zeatin.KT-Kinetin.
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This work is a part of cooperative research project between CNCBD and Rockefeller Foundation.
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Wang, G., Hsia, C., Chi, J. et al. Plant regeneration from cultured protoplasts of a glutinous rice. Cell Res 1, 17–21 (1990). https://doi.org/10.1038/cr.1990.3
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DOI: https://doi.org/10.1038/cr.1990.3
