Abstract
We characterized the microbiologically mediated oxidative precipitation of Fe(II) from coalmine-derived acidic mine drainage (AMD) along flow-paths at two sites in northern Pennsylvania. At the Gum Boot site, dissolved Fe(II) was efficiently removed from AMD whereas minimal Fe(II) removal occurred at the Fridays-2 site. Neither site received human intervention to treat the AMD. Culturable Fe(II) oxidizing bacteria were most abundant at sampling locations along the AMD flow path corresponding to greatest Fe(II) removal and where overlying water contained abundant dissolved O2. Rates of Fe(II) oxidation determined in laboratory-based sediment incubations were also greatest at these sampling locations. Ribosomal RNA intergenic spacer analysis and sequencing of partial 16S rRNA genes recovered from sediment bacterial communities revealed similarities among populations at points receiving regular inputs of Fe(II)-rich AMD and provided evidence for the presence of bacterial lineages capable of Fe(II) oxidation. A notable difference between bacterial communities at the two sites was the abundance of Chloroflexi-affiliated 16S rRNA gene sequences in clone libraries derived from the Gum Boot sediments. Our results suggest that inexpensive and reliable AMD treatment strategies can be implemented by mimicking the conditions present at the Gum Boot field site.
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Acknowledgements
We thank Pam Milavec and Jon Smoyer from the Pennsylvania Department of Environmental Protection for directing us to the Gum Boot and Fridays-2 systems. We thank Dr D Barrie Johnson from the University of Wales, Bangor for advice in preparing the solid medium for iron-oxidizing bacteria. We thank Richard Unz for critically reviewing the article and providing valuable suggestions. This work was supported by the Pennsylvania Department of Environmental Protection, Bureau of Abandoned Mine Reclamation, the Pennsylvania Water Resources Research Center and the Center for Environmental Chemistry and Geochemistry at the Pennsylvania State University. ML was supported by the National Science Foundation under Grant No. CHE-0431328 and the US Department of Energy, Biological and Environmental Research (BER).
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Senko, J., Wanjugi, P., Lucas, M. et al. Characterization of Fe(II) oxidizing bacterial activities and communities at two acidic Appalachian coalmine drainage-impacted sites. ISME J 2, 1134–1145 (2008). https://doi.org/10.1038/ismej.2008.60
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