Abstract
Although uncultured microorganisms have important roles in ecosystems, their ecophysiology in situ remains elusive owing to the difficulty of obtaining live cells from their natural habitats. In this study, we employed a novel magnetic nanoparticle-mediated isolation (MMI) method to recover metabolically active cells of a group of previously uncultured phenol degraders, Burkholderiales spp., from coking plant wastewater biosludge; five other culturable phenol degraders—Rhodococcus sp., Chryseobacterium sp. and three different Pseudomonas spp.—were also isolated from the same biosludge using traditional methods. The kinetics of phenol degradation by MMI-recovered cells (MRCs) was similar to that of the original sludge. Stable isotope probing (SIP) and pyrosequencing of the 16S rRNA from the ‘heavy’ DNA (13C-DNA) fractions indicated that Burkholderiales spp. were the key phenol degraders in situ in the biosludge, consistent with the results of MRCs. Single-cell Raman micro-spectroscopy was applied to probe individual bacteria in the MRCs obtained from the SIP experiment and showed that 79% of them were fully 13C-labelled. Biolog assays on the MRCs revealed the impact of various carbon and nitrogen substrates on the efficiency of phenol degradation in the wastewater treatment plant biosludge. Specifically, hydroxylamine, a metabolite of ammonia oxidisation, but not nitrite, nitrate or ammonia, inhibited phenol degradation in the biosludge. Our results provided a novel insight into the occasional abrupt failure events that occur in the wastewater treatment plant. This study demonstrated that MMI is a powerful tool to recover live and functional cells in situ from a complex microbial community to enable further characterisation of their physiology.
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Acknowledgements
We thank EU ECOWATER project (RFCR-CT-2010-00010) and EPSRC Grant EP/H04986X/1 for financial support. WEH and JX acknowledge the support from the Soil Microbiota Program from the Chinese Academy of Sciences (XDB15040100) and Methodology Innovation Program from the Ministry of Science and Technology of China (MOST 2011IM030100). YC is supported by NERC (NE/H016236/1) and GBMF (GBMF3303). The authors thank Andrew Fairburn in Sheffield and Cunpei Bo and Fei Teng in CAS for technical support.
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WEH, DaZ and EA designed the research. DaZ, WEH, JPB, DiZ, HL, BJ, GL and YC performed the experiments. DaZ and WEH analysed data. YW, SH and JX undertook computational analysis. WEH, DaZ, PAD and YC wrote the paper.
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Zhang, D., Berry, J., Zhu, D. et al. Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community. ISME J 9, 603–614 (2015). https://doi.org/10.1038/ismej.2014.161
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DOI: https://doi.org/10.1038/ismej.2014.161
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