Abstract
A new bafilomycin-type macrolide, named hygrobafilomycin (6), was isolated by a bioassay-guided selection and fractionation from a terrestrial actinomycete, Streptomyces varsoviensis, along with three known derivatives, bafilomycin D (3), C1 (4) and C2 (5). The structure of hygrobafilomycin was fully established by MS and NMR analyses, revealing a hygrolidin–bafilomycin hybrid with an unusual monoalkylmaleic anhydride moiety. Hygrobafilomycin (6) shows strong antifungal, antiproliferative and cytotoxic activities. In a panel of 40 tumor cell lines, compound 6 shows high cytotoxic potency (mean IC50=5.3 n).
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Acknowledgements
Financial support by the BMBF (FKZ 0315153) is gratefully acknowledged. We thank Heike Heinecke and Ulrike Valentin for technical assistance, Franziska Rhein for NMR measurements, Andrea Perner for MS measurements, Jutta Fehr and Roger Vollmer for cytotoxicity assays, Maria-Gabriele Schwinger and Christiane Weigel for antimicrobial assays.
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Tchize Ndejouong, B., Sattler, I., Maier, A. et al. Hygrobafilomycin, a cytotoxic and antifungal macrolide bearing a unique monoalkylmaleic anhydride moiety, from Streptomyces varsoviensis. J Antibiot 63, 359–363 (2010). https://doi.org/10.1038/ja.2010.52
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DOI: https://doi.org/10.1038/ja.2010.52
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