Bacillus species have the ability to secrete lipopeptide antibiotics under both in vivo and in vitro conditions.1, 2, 3, 4 Bacillus lipopeptides are versatile antibiotics composed mainly of three families—iturins, plipastatins (or fengcyins) and surfactins. Iturins and plipastatins are fungicides and have potential utilization in the biological control of fungal plant pathogens.5 Marine-derived microorganisms have arisen as a focus of research during recent decades, and numerous novel metabolites with excellent biological activity have been reported.6, 7 In the present work, compound 1 was isolated and purified from the fermentation broth of a marine sediment-derived B. amyloliquefaciens SH-B74. The structure of compound 1 was characterized by one-dimensional and two-dimensional NMR spectroscopy as well as tandem mass spectrometry (MS/MS) analysis. Finally, the antagonistic activities of compound 1 to several plant pathogens were also evaluated in vitro by the paper disc-agar diffusion assay.

Compound 1 was obtained as a white amorphous powder. The HRESI MS spectrum of compound 1 showed m/z 1057.5177 ([M+Na]+) for chemical formula C47H74N10NaO16, which was calculated for 1057.5182. The result could be further demonstrated by the full mass spectrum of compound 1, which showed m/z 1035.49 ([M+H]+) and 1057.48 ([M+Na]+).

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