Genome-based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in a novel strain taxonomically close to the genus Salinispora

Members of the genus Salinispora are obligate marine actinomycetes of the family Micromonosporaceae, and require sodium-enriched medium for growth.¹ This genus has attracted attention from researchers seeking novel secondary metabolites, because it represents promising sources of such molecules.^{2, 3} However, it is difficult to isolate novel strains within or closely related to the genus Salinispora; only three species, Salinispora arenicola, Salinispora pacifica and Salinispora tropica, have been discovered during the past decade.

In our survey of actinomycetal inhabitants of mangrove forests, we isolated a novel strain, designated NBRC 107566, from the rhizosphere of a mangrove growing on Iriomote Island, Okinawa, Japan. The 16S rRNA gene of the strain showed high sequence similarities to those of S. arenicola CNH-643^T (98.28%), Micromonospora pattaloongensis TJ2-2^T (98.08%), S. pacifica CNR-114^T (98.07%) and S. tropica CNB-440^T (98.07%). Thus, to assess the potential of NBRC 107566 as a secondary metabolite producer, we performed whole-genome shotgun sequencing and examined type-I polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters, which are involved in the major secondary metabolite-synthetic pathways in actinomycetes. The taxonomic study of this strain will be reported elsewhere.

Log in or create a free account to read this content

Gain free access to this article, as well as selected content from this journal and more on nature.com

Access through your institution

or

Sign in or create an account

Continue with Google

Continue with ORCiD