Figure 6

Regulatory T cells in the liver of fibrotic or fibrotic combined with tumor mice. Intra-spleen inoculation of ML-1 established tumor nodule formation in the liver of BALB/c mice. TAA was used to induce fibrosis. CD4⁺ or CD8⁺ T cells were depleted by intraperitoneal injection of anti-CD4 antibody (clone GK 1.5) or anti-CD8 antibody (clone 2.43) every 7 days from 1 week before ML1 implantation. (a) Tumor nodule formation in CD4⁺ T-depleted mice. (b) Tumor nodule formation in CD8⁺ T-depleted mice. (c) Tumor-infiltrating CD8⁺ T cells in normal or CD4⁺ T-cell depleted mice. (d) The percentages of Foxp3⁺/CD3⁺ T cells in intrahepatic leukocytes were analyzed by flow cytometry. The cells isolated from fibrosis combined with hepatoma-treated mice were stained with anti-CD3 and anti-Foxp3. (e) Immunofluroscence staining of Foxp3⁺ (red), CD3⁺ (green) and DAPI (blue) in mice liver. The DAPI condensed parts are considered as tumor area (white dotted line). The images are representative of six mice in each group. (f) Shows the quantitative result in e.