Figure 3 | Laboratory Investigation

Figure 3

From: Anti-Müllerian hormone inhibits growth of AMH type II receptor-positive human ovarian granulosa cell tumor cells by activating apoptosis

Figure 3

Cell number and proliferation assays on KGN (a, b) and two primary cell cultures (c) after AMH treatments. Cells were treated with indicated doses of AMH for 1–10 days (d) before MTT-based or BrdU incorporation assays. 10% FFCS was used as a control, and 1% FFCS as a control for growth factor deprivation. (a) MTT data on the relative number of viable KGN cells is presented as mean±s.d. of three independent experiments performed in triplicate; the relative cell numbers are shown compared with control. (b) The proportion (%) of proliferative, ie, BrdU-positive, cells is presented as mean±s.d. of three independent experiments; total of 2000–3000 cells was counted for each dose and incubation period. (c) MTT data on the number of viable cells of two primary cell cultures are presented as mean±s.d. of one independent experiment performed in triplicate, treated for 2 days (case 1, white columns) or for 4 days (case 2, black columns). Asterisks indicate significant difference with P<0.05 when compared with control experiment (AMH 0 μg/ml).

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