Figure 4 | Laboratory Investigation

Figure 4

From: Anti-Müllerian hormone inhibits growth of AMH type II receptor-positive human ovarian granulosa cell tumor cells by activating apoptosis

Figure 4

AMH activates Smad1/5, caspase-3, and apoptosis in KGN cells. (a) Representative western blotting results of an experiment performed in triplicate, showing the effect of increasing AMH doses on protein levels of Smad1/5, cleaved (activated) caspase-3, Bcl2, cyclinD2, and β-actin as an internal control. (b) Caspase-3/7 activation assay following increasing doses of AMH for 1–3 days, presenting fold activation relative to control. (c) Number of apoptotic cells analyzed by nuclear morphology following DAPI staining. In b and c the data are presented as mean±s.d. of three independent experiments performed in triplicate. Asterisks in b and c indicate significant difference with P<0.05 when compared with control experiment (AMH 0 μg/ml).

Back to article page