Table 1 Antibodies and primers used in the expression analyses
Factor size | Antibody | Dilution: WB/IHC | Primers 5′–3′ | PCR product | |
|---|---|---|---|---|---|
AMH | Goat IgG, sc-6886, Santa Cruz Biotechnology | –/1:100 | qPCR | F: CGCCTGGTGGTCCTACAC R: GAACCTCAGCGAGGGTGTT | 60 bp |
AMHRII | Mouse monoclonal IgG | 1:5000/1:50 | qPCR | F: TGTGTTTCTCCCAGGTAATCCG R: AATGTGGTCGTGCTGTAGGC | 164 bp |
PCR | F: TTTGGGGCTTTGGGCATTAC R: GATGCCGAGACAGTGAT | 238 bp | |||
ALK2 | PCR | F: TTAAAAGGCGCAACCAAGA R: CGTACAACGATCCCATTTCA | 423 bp | ||
ALK3 | PCR | F: TTTATGGCACCCAAGGAAAG R: TGGTATTCAAGGGCACATCA | 156 bp | ||
ALK6 | PCR | F: CTCAGGGAGCGACCTGGGCA R: GCGGCCCCAAATGCAGGGAT | 437 bp | ||
Smad1 | Mouse IgG ab55437, Abcam | 1:1000/1:50 | PCR | F: GCGGCATATTGGAAAAGGAG R: CCTGGGGCCATTTAAGAT | 429 bp |
Smad5 | Rabbit IgG, ab40771, Abcam | 1:2500/1:50 | PCR | F: GCGAAAAGGAAGCTGTTG R: ACCTTGTTTCCAGCCCA | 320 bp |
Beta-actin | qPCR | F: CTGACGGCCAGGTCATCAC R: CAGACAGCACTGTGTTGGC | 174 bp | ||
Phospho-Smad1/5 | Rabbit IgG, #9511S, Cell Signaling Technology | 1:2500/1:100 | |||
Cleaved caspase-3 | Rabbit IgG, #9661L, Cell Signaling Technology | 1:500/– | |||
Bcl2 | Mouse IgG, MO887, DAKO Denmark | 1:500/– | |||
Cyclin D2 | Rabbit IgG, sc598, Santa Cruz Biotechnology | 1:1000/– | |||
