Figure 1
Caspase-11 deficiency sensitizes mice to dextran sodium sulfate (DSS)–induced morbidity and lethality. (a) Schematic of the design of the survival study and the studies for the collection of serum and colon samples. In general, mice were fed a 2 or 4% DSS solution in drinking water for 5 days, followed by normal drinking water until the end of the experiment. (b) Kaplan–Meier survival plot of C57BL/6J, caspase-11−/− (C11−/−), and caspase-1−/−/11−/− (C1−/−/11−/−) mice induced with 4% DSS (n=10 per group). (c) The percentage of body weight and (d) the clinical score of C57BL/6J, C11−/−, and C1−/−/11−/− mice induced with 2% DSS. Data depict the mean±s.e.m. (n=10 per group). Statistical significance between DSS-treated C57BL/6J and C11−/− mice is indicated. (e) Macroscopic pictures and (f) graphic presentation of the colon length of C57BL/6J, C11−/− and C1−/−/11−/− mice without (0% DSS) or 10 days after induction with 2% DSS. Data in f depict individual replicates with mean; C57BL/6J mice (0% DSS, n=8; 2%, n=7), C11−/− mice (0% DSS, n=6; 2%, n=11), and C1−/−/11 mice (0% DSS, n=4; 2% DSS, n=6). Statistical significance between DSS-treated C57BL/6J, C11−/−, and C1−/−/11−/− mice is indicated. *P<0.05; **P<0.01; ***P<0.001.
