Figure 4
Increased colonic expression of CCL5 correlates with higher inflammatory cell infiltration in caspase-11−/− mice after dextran sodium sulfate (DSS) treatment. (a–c) Production of interleukin-6 (IL-6; a), KC (b), and CCL5 (c) in the colon of C57BL/6J, caspase-11−/− (C11−/−) and caspase-1−/−/11−/− (C1−/−/11−/−) mice without (0% DSS) or 10 days after induction with 2% DSS. Production of (d) IL-6, (e) KC, and (f) CCL5 in the colon of C57BL/6J and C11−/− mice without (0% DSS) or 7 days after induction with 4% DSS. Data depict the individual replicates with mean; C57BL/6J mice (0% DSS, n=8; 2%, n=7; and 4%, n=10), C11−/− mice (0% DSS, n=6; 2%, n=11; and 4%, n=10), and C1−/−/11−/− mice (0% DSS, n=4; and 2%, n=6). Statistical significance between DSS-treated C57BL/6J, C11−/− and C1−/−/11−/− mice is indicated. *P<0.05; **P<0.01; ***P<0.001; n.s., not significant. (g,h) Photomicrographs of macrophage (F4/80) and neutrophil (Ly6G) staining on representative colon sections of DSS-treated C57BL/6J and C11−/− mice without (0% DSS; n=4 per group) or 10 days after induction with 2% DSS (n=4 per group). Bar=100 μm.
