Figure 1
From: Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation
The influence of pharmacological HIF stabilization on the development of DSS colitis associated pathology. Groups were treated at 48 h intervals with PHDi (5 mg kg−1 AKB-4924) or vehicle (veh) (cyclodextrin) from day 0. (a) Weight change (%) from initial weight and pathological shortening and thickening of the colon expressed as weight (mg):length (cm) (Vehicle n=6, PHDi n=6, DSS+Veh n=11, DSS+PHDi n=11). (b) Representative H&E-stained colon sections at 10 × magnification from healthy treated with vehicle or DSS-treated animals treated with vehicle or PHDi. (c) Sections were scored to provide inflammation and epithelial injury scores. (d) Endoscopic scores from day 4 to 15 post DSS for animals treated with vehicle or PHDi. (e) % weight change from initial weight, (f) representative H&E-stained colon sections (10x) and (g) inflammation and epithelial injury scores on day 15 post DSS for animals treated with vehicle or PHDi. Data expressed as mean ±s.e.m., n=6-11, (a, e) two-way ANOVA, (c, d, g) one-way ANOVA, *P<0.05 **P<0.01. Scale bar, 100 μm. ANOVA, analysis of variance; DSS, dextran sulfate sodium; H&E, hematoxylin and eosin; HIF, hypoxia-inducible factor-1α.
