Figure 6
From: Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation
The influence of PHDi on Th17 responses in the Citrobacter rodentium model of infectious colitis. Flow cytometric analysis of intracellular cytokine production from LPL re-stimulated in vitro. (a) Representative dot plots of LPL CD3+CD4+ T cells producing IL-17 and IFN-γ or IL-22 from naive (N) uninfected or C. rodentium-infected mice receiving vehicle (Veh) or PHDi (AKB-4924 5mg kg−1) 14 days post infection. (b) % live CD3+CD4+ T cells expressing IFN-γ, IL-17 and IL-22 or (c) IL-17/IFN-γ and IL-17/IL-22 double-expression. (d) Real-time PCR analysis of il17a and il22 expression in isolated LPL populations. (e) Changes in mucosal thickening (f) spleen weight and (g) histological colitis score in C. rodentium-infected animals with vehicle or PHDi treatment, compared with naive animals. (h) Representative H&E-stained histological images of colons from uninfected naive animals, or C. rodentium infected animals treated with vehicle or PHDi. Data expressed as mean ±s.e.m., (n=6 per group). **P<0.01; ***P<0.005. One-way ANOVA, *P<0.05. ANOVA, analysis of variance.
