Table 2 Comparison of mutations found and overall performance between cytology slides and supernatants
Microdissected cytology slides | Cytocentrifugation supernatant | Comparison of mutations | ||||
|---|---|---|---|---|---|---|
Sample | Cytological diagnosis | KRAS mutations a | LOH | KRAS mutations a | LOH | between found in slides versus supernatant |
1 | Adenocarcinoma | KRAS 12V low clonality | No LOH | KRAS 12V low clonality | 22q low clonality | Additional mutations detected |
2 | Adenocarcinoma | KRAS 12V low clonality | 5q high clonality, 3p low clonality, 17p low clonality | KRAS 12V low clonality | 5q high clonality, 3p low clonality, 9p low clonality, 17p low clonality, 17q low clonality | Additional mutations detected |
3 | Adenocarcinoma | No mutations | 17p low clonality, 21q low clonality | No mutations | 17p high clonality, 21q high clonality, 5q low clonality | Higher mutation clonality and additional mutations detected |
4 | Adenocarcinoma | No mutations | No LOH | KRAS 12R low clonality | 17p high clonality, 5q low clonality | Additional mutations detected |
5 | Adenocarcinoma | KRAS 12D low clonality | 1p low clonality, 22q low clonality | KRAS 12D high clonality | 1p high clonality, 9p high clonality, 17q high clonality, 10q low clonality | Higher mutation clonality and additional mutations detected |
6 | Mucinous | Not tested | Not tested | No mutations | 9q high clonality | N/A |
7b | Mucinous | KRAS 12D high clonality | 9p high clonality, 10q high clonality, 3p low clonality | KRAS 12D high clonality | 9p high clonality, 10q high clonality, 3p low clonality, 5q low clonality | Additional mutations detected |
8b | Mucinous | KRAS 12D high clonality | 9p high clonality, 10q high clonality, 3p low clonality | KRAS 12D high clonality, | 9p high clonality, 10q high clonality, 3p low clonality, 5q low clonality | Additional mutations detected |
9 | Mucinous | No mutations | 9p high clonality | No mutations | 9p high clonality | Equivalent |
10 | Mucinous | Not tested | Not tested | KRAS 12V low clonality | No LOH | N/A |
11 | Neuroendocrine | No mutations | 3p high clonality, 9p low clonality | No mutations | 3p high clonality, 9p high clonality | Equivalent |
12 | Pancreatitis | No mutations | No LOH | No mutations | No LOH | Equivalent |
13 | Pancreatitis | No mutations | No LOH | No mutations | No LOH | Equivalent |
14 | Pancreatitis | No mutations | No LOH | No mutations | No LOH | Equivalent |
15 | Pancreatitis | No mutations | No LOH | No mutations | No LOH | Equivalent |
16 | Pancreatitis | No mutations | Failed PCR | No mutations | No LOH | Equivalent |
17 | Negative | No mutations | No LOH | No mutations | No LOH | Equivalent |
18 | Negative | Not tested | Not tested | No mutations | No LOH | N/A |
19 | Negative | No mutations | No LOH | No mutations | No LOH | Equivalent |
20 | Negative | No mutations | No LOH | No mutations | No LOH | Equivalent |
21 | Suboptimal | No mutations | No LOH | No mutations | No LOH | Equivalent |
22 | Suboptimal | Failed PCR | Failed PCR | No mutations | No LOH | Improved performance |
23 | Suboptimal | KRAS 12C low clonality | Failed PCR | KRAS 12C low clonality, KRAS 12V low clonality | No LOH | Improved performance and additional mutations detected |
