Extended Data Figure 4: KDU731 inhibits C. parvum Nluc parasites in vitro and in vivo.
From: A Cryptosporidium PI(4)K inhibitor is a drug candidate for cryptosporidiosis
a, EC50 determination of KDU731 against UGA1 Nluc transgenic parasites grown in HCT-8 cultures using luciferase activity as read out. Representative data are shown, three technical replicates. b, Mice (n = 5) were infected with 10,000 UGA1 Nluc oocysts and treated orally 3 days after infection with 1, 5, or 10 mg per kg (body weight) KDU731 for 1 week. Faecal oocyst load was determined by measuring parasite luciferase activity (b) or parasite DNA by qPCR (c) in faeces pooled from entire cage of five mice (20 mg faeces for Nluc and 100 mg for PCR assay). b, c, Means for three technical replicates are shown. Error bars, s.d. Pooled Nluc experiments for vehicle and 10 mg per kg (body weight) dose were repeated in three biological replicates and a representative result is shown.
