Supplementary Figure 2: ERCC1 and MUS81 cellular localisation. | Nature Cell Biology

Supplementary Figure 2: ERCC1 and MUS81 cellular localisation.

From: ERCC1 and MUS81–EME1 promote sister chromatid separation by processing late replication intermediates at common fragile sites during mitosis

Supplementary Figure 2

(a) ERCC1 localisation on mitotic chromosomes is independent of FANC pathway proficiency. FANCD2 (red in the merged image) and ERCC1 (green in the merged image) are shown from left to right in FANCC-proficient (top panels) and FANCC-deficient (bottom panels) cells. DNA (DAPI) is blue in the merged image. (b) MUS81 localisation on mitotic chromosomes is independent of FANC pathway proficiency. FANCD2 (red in the merged image) and MUS81 (green in the merged image) are shown from left to right in FANCC-proficient (top panels) and FANCC-deficient (bottom panels) cells. DNA (DAPI) is blue in the merged image. (c) Sub-cellular localisation of MUS81 (upper panels, green in the merged image) and ERCC1 (lower panels, green in the merged image) in untreated HeLa cells. Fibrillarin (red in the merged image) was used as a nucleolar marker. Scale bars = 10 μm.

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