Supplementary Figure 2: Activation of ATM promotes the degradation of ARF by disrupting the ARF-NPM/B23 complex. | Nature Cell Biology

Supplementary Figure 2: Activation of ATM promotes the degradation of ARF by disrupting the ARF-NPM/B23 complex.

From: Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer

Supplementary Figure 2

a. TRIP12/ULF protein levels remain unchanged after ATMi or Doxorubicin treatment in H1299 cells. Actin serves as loading control. ATMi = Ku55933 addition. b. IB analysis in siNPM/B23 H1299 cells showing downregulation of p14ARF denoting the significance of NPM/B23 in p14ARF stabilization. c. Nucleolar changes after exposure to ATM inhibition and Doxorubicine. H1299 cells were treated with doxorubicine (2uM) and the ATM inhibitor Ku55933 (10uM) for 24h. Untreated and treated cells were fixed and subjected to immunofluorescence staining with antibodies against the indicated nucleolar markers. Fibrillarin and UBF nucleolar cap structures are indicated by arrowheads. Fluorescence signals were analyzed by CLSM. Dox = Doxorubicin, ATMi = Ku55933 addition.

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