Supplementary Figure 7: ATM abrogation is inversely related with ARF expression in human cancer. | Nature Cell Biology

Supplementary Figure 7: ATM abrogation is inversely related with ARF expression in human cancer.

From: Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer

Supplementary Figure 7

a. Cumulative data (histograms) depicting the distribution of the lung cancer cases examined in the current work, according to the status of ATM, p-ATM, Chk2, p14ARF and p16INK4A66,67,68,69. b. Meta-analysis regarding alterations in the ATM gene and its protein product levels. Histograms represent the percentage of ATM alterations in the corresponding neoplasias. The red bar is the result of the current study. d. Phosphorylated I-2 PP1 regulatory subunit expression corroborates with ATM status in human lung carcinomas. Cases with normal ATM demonstrate by IB analysis increased expression of the phosphorylated I-2 PP1 regulatory subunit (pI-2). The reduced intensity of the slower migrating pI-2 band upon calf intestinal phosphatase (CIP) treatment of the blots denotes the specificity of the antibody used for the detection of the phosphorylated form of the I-2 subunit. In contrast, cases with low ATM levels do not show any levels of the pI-2 subunit14,30,33. Actin serves as loading control.

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