Supplementary Figure 7: Analysis of myo-II phosphorylation mutants in neuroepithelium morphogenesis.

(A-B) SqhEE, asp (A) and SqhAA, asp (B) mutant brains stained with Armadillo to visualize the NE and Hoescht (red in merged panels) to label DNA. Low magnification images showing the anterior surface (top view, left), and a cross-section (middle) view of the third instar larval brains. A higher magnification of the NE is shown in the NE overview panel (right). Scale bar: 30 μm in top and cross section views and 10 μm in overview. (C) Quantification of the NE length in the apical-basal axis (bars) and the length of the basal process of dividing nuclei (dot plots) in SqhEE (n = 28 and n = 25 cells for cell length and basal process length respectively), SqhEE, asp (n = 66 and n = 38 for cell length and basal process length respectively), SqhAA (n = 57 and n = 20 cells for cell length and basal process length respectively) and SqhAA, asp (n = 100 and n = 48 cells for cell length and basal process length respectively). This plot should be compared to plot shown in Fig. 4d for WT and asp. Statistical significance was assessed by a two-tailed unpaired t-Test.