Supplementary Figure 4

Uncropped Western blots referring to main figures 1.a, 1.b, 3.b-f. Regions shown in main figures are marked with red squares. All Western blots were named according to the main figure to which they relate to. (Fig. 1a) Distribution analysis of growth cone fractionation showing specific enrichment of GAP43, axonal growth cone marker. (Fig. 1b) Enrichment of R-SNAREs in growth cone membrane fraction compared to total brain homogenate. GAP43 and nucleoporins were used as positive and negative control, respectively. (Fig. 3b) Western blot showing Stx1 immunoprecipitation from embryonic and adult brain, blotted for Stx1 on the left, and reblotted on the same membrane for Sec22b on the right. (Fig. 3c) Enrichment of SNAPs in growth cone membrane fraction compared to total brain homogenate. (Fig. 3d) Western blot showing SNAP25 immunoprecipitation from embryonic and adult brain, blotted for SNAP25 and VAMP2, and Sec22b. Membrane on the left shows SNAP25 and VAMP2, and membrane on the right from a separate gel done in the same way shows SNAP-25 and Sec22b. (Fig. 3e) Western blot showing SNAP23 immunoprecipitation from embryonic and adult brain, blotted for SNAP23 and VAMP2 on the left, and reblotted on the same membrane for Sec22b on the right. (Fig. 3f) Western blot showing SNAP29 immunoprecipitation from embryonic and adult brain, blotted for Stx1, Sec 22b and VAMP2 on the left, and membrane on the right from a separate gel done in the same way shows SNAP-29.