Supplementary Figure 5

Uncropped Western blots referring to main figures 6.a-c, 6.e. Regions shown in main figures are marked with red squares. All Western blots were named according to the main figure to which they relate for simplicity. (Fig. 6a) Western blot showing GFP-Sso1 and GFP-Sec22p immunoprecipitation from yeast, blotted for GFP, Sso1 and Sec22p. (Fig. 6b) Western blot showing GFP-Sso1 expression over time from plasmid under tetracycline-repressible promoter (pAP87-GFP-Sso1) stimulated with doxycycline. Membrane is blotted for Sec22. (Fig. 6c) Western blot showing GFP immunoprecipitation from GFP-Sso1 and GFP-Vam7 expressing yeast cells, blotted for Sec22. (Fig. 6e) Western blot showing GFP immunoprecipitation from GFP-Osh2 and GFP-Osh3 expressing yeast cells, blotted for GFP, Sso1 and Sec22p.