Supplementary Figure 6: Analysis of gene expression changes in hESC-HSPCs on AM580 treatment shows partial conversion to definitive HSC transcriptome.
From: Medial HOXA genes demarcate haematopoietic stem cell fate during human development
(A) RNA-seq genome browser screenshot of the HOXA cluster of day 12 EB and FL derived immunophenotypic HSPCS that were treated with AM580 for 6 days (6 days of treatment and 6 additional days in culture). (B) Representative genes upregulated by AM580 treatment at day 6, shown at day 12 as compared to FL-HSPCs. (C) Representative genes upregulated by HOXA7 shRNA knockdown (see Fig. 4l) shown in day 12 EB derived cells treated with AM580 (6 days of treatment and 6 additional days in culture) as compared to FL-HSPCs (showing mean from 2 independent experiments, values used to generate graphs in B and C can be found in Supplementary Table 5 and GEO database GSE76685).
