Supplementary Figure 1: FL derived, but not hESC derived haematopoietic cells can reconstitute human HSPC compartment in recipient BM.

(A) Schematic of transplantation of CD34⁺ cells into irradiated NSG mice. (B) NSG mice were transplanted with CD34⁺ cells from hESCs (EB and EB-OP9) and fetal liver (FL or FL-OP9) and human engraftment in the BM assessed at 12 weeks for CD45⁺CD34⁺CD38⁻CD90⁺ immunophenotypic HSPCs. Results shown are from representative animals for each group of transplanted mice (5 mice transplanted with EB cells, 4 with EB-OP9 cells, 4 with FL cells, and 3 with FL-OP9 cells).