Supplementary Figure 2: LIFR is regulated via DNA methylation in breast cancer patients likely to recur. | Nature Cell Biology

Supplementary Figure 2: LIFR is regulated via DNA methylation in breast cancer patients likely to recur.

From: Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow

Supplementary Figure 2: LIFR is regulated via DNA methylation in breast cancer patients likely to recur.

(a) Western blot for LIFR in MCF7 whole cell lysates following lentiviral knockdown. MCF7 non-silencing control (MCF7NSC) and MCF7shRNA #3 cells were used in all remaining experiments. Vinculin = loading control. (b) PGK1 mRNA levels for MCF7NSC cells cultured in normoxia (Nx) or hypoxia (Hx; 0.5% oxygen) for 24 h. 3 technical replicates from a single experiment representative of 2 independent experiments. (c) LIFR mRNA levels in SUM159 human breast cancer cells following 24 h culture in normoxia (Nx) or hypoxia (Hx; 0.5% oxygen). 3 technical replicates from a single experiment. (d) Correlation of LIFR mRNA levels (y-axis) or (e) STAT3 mRNA levels (y-axis) and LIFR DNA methylation (x-axis) in patients with invasive breast carcinoma (TCGA Provisional dataset, n = 1,104 patients). Pearson Correlation and Spearman Correlation. (f) LIFR mRNA levels (n = 850 patients disease free; n = 68 patients recurred/progressed) and (g) LIFR DNA methylation (n = 575 patients disease free; n = 51 patients recurred/progressed) in patients with invasive breast carcinoma (TCGA Provisional dataset). Student’s unpaired t-test. Source data for Suppl. 2b available in Supplementary Table 1. Graphs represent the mean/group and error bars represent standard error of the mean (SEM). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001.

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