Supplementary Figure 3: LIF inhibits proliferation in breast cancer cells with low metastatic potential. | Nature Cell Biology

Supplementary Figure 3: LIF inhibits proliferation in breast cancer cells with low metastatic potential.

From: Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow

Supplementary Figure 3: LIF inhibits proliferation in breast cancer cells with low metastatic potential.

(a–c) XTT assay to determine proliferation of MCF7, MDA-MB-231b, and 4T1BM2 cancer cells in response to recombinant LIF (50 ng ml−1) or TGF-β (5 ng ml−1) over 3 days. Two-Way ANOVA with Tukey’s multiple comparisons test. n = 3 biological replicates/day. (d,e) SOCS3 mRNA levels in MCF7 and MDA-MB-231b cells following 1 or 6 h treatment with recombinant LIF (0–100 ng ml−1). One-Way ANOVA with Sidak’s multiple comparisons test. n = 3 biological replicates, each being an average from 3 independent experiments. Source data for Suppl. 3a–c available in Supplementary Table 1. Graphs represent the mean/group and error bars represent standard error of the mean (SEM). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001.

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